Sentence examples for markers the cells from inspiring English sources

After immunostaining of the surface markers, the cells were fixed 10 min with 3% formaldehyde in PBS.

To analyze the amounts of intracytoplasmic and nuclear markers, the cells were permeabilized with 5 μl of 0.1% Triton X-100 for 30 min before the addition of specific primary antibodies.

For standard MSC surface markers, the cells were strongly positive for CD90 (99.9% in TCPS versus 99.7% in ECM) and CD105 (96.5% in TCPS versus 81.5% in ECM), whereas the cells were negative for CD34 (0.5% in TCPS versus 0.8% in ECM) and CD45 (1.6% in TCPS versus 1.4% in ECM).

For the identification of surface markers, the cells were incubated for 20 min with flow cytometry buffer (1% BSA in PBS) on ice and were subsequently incubated for 30 min with primary and secondary antibodies in the dark on ice at 4 °C.

In addition to these markers, the cells in the cluster express several lymph gland hemocyte-specific markers like ZCL2897 (Jung et al., 2005), and are Serpent (Srp) and dorothy-GFP (Honti et al., 2010) positive, even some of them are lineage traced for collier (Crozatier et al., 2004).

After the first passage of hypoxic differentiated cells (cells positive to SMCs markers), the obtained cells were expanded under two conditions.

Only 21% of cells expressed both markers; the remaining cells expressed either one or neither.

These smaller foreground regions represent the markers of the cells as shown in Figure 6f.

Use in plant bioimaging There is an increasing application of QD as markers for the cells or cell walls (CWs) in plant science.

Attention has emphasized markers identifying the cells with self-renewal and tumor-reinitiating capacities.

In contrast to lymphocyte markers, the dendritic cell marker CD1a showed no association with DSS, possibly due to the low number of tumors containing CD1a+ cells.