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Markers that maintained the expected pattern of polymorphisms, were then used to screen the entire RIL population.
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NANOG is an early transcription factor and pluripotent marker that maintains the self-renewal of embryonic and mesenchymal stem cells [ 8].
All these in vivo data illustrated that in cells lacking laforin there was an induction of ER-stress markers and impaired proteasomal function that maintained these cells are under permanent conditions of ER-stress.
These markers were chosen from a comprehensive panel of 25 markers used in our previously study (Abd et-Rehim et al, 2005) as the minimum number of markers that can maintain class membership and identify the same molecular classes (Green et al, 2013).
We next asked if the coincident presence of nestin and GR is a stable phenotypic marker that is maintained after explantation of primary cells in tissue culture.
Since GLUT5 conferred a rather slow growth on synthetic media (about five days until colonies appeared on agar plates; see Fig. 1a), we generated plasmids with dominant markers that can be maintained on rich media (YEP) for further functional studies.
These metastases possess polarity markers and a re-epithelialization that maintains junctional protein expression.
The methodology is based on a high-definition video camera, plus LED lights and laser scale markers, mounted on a "flying array" that maintains itself above the seabed grounded by a length of chain, thus causing minimal damage.
These data suggest that cultured PGCs are immortalized cells that maintain expression of both surface markers and PGC-specific genes.
Using latent class trajectory models to identify distinct groups with similar underlying patterns of longitudinal weight change, we found lower levels of diabetes markers in classes that maintained weight, lost weight or switched from weight gain to weight loss.
These conflicting data, when combined with the uncertain biological role of CD133, highlight the need for additional distinguishing markers that are directly involved in maintaining the functional properties of the putative cancer stem cell population [ 35].
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