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The physico-chemical traits like amylose content, chalkiness, ASV did not show any association among themselves and with other traits clearly indicating the oligogenic nature of the traits.> In the present study, 203 of the 552 microsatellite markers tested produced polymorphic and scorable bands (42.12 % polymorphism) between the parents Basmati370 and Jaya.
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A GWAS method testing individual SNP effects was chosen because previous studies had demonstrated that single-marker tests produced similar or even greater power than haplotype-based approaches [ 35, 36].
Overall, 146 out of the 209 (69.9%) markers tested on G. armourianum produced successful assays.
From the 30 potential microsatellite markers tested, 18 failed to produce a reproducible polymorphic pattern.
Out of 14 microsatellite markers tested, only UMN2303 and SRYM18 produced male-specific products.
When used to screen all BAC superpools from the library, 37 pairs of primers (17% of all markers tested) failed to amplify, even though they produced amplification bands when tested against melon genomic DNA.
SRAP markers produced a significantly greater number of variable fragments per locus (≥1.25 polymorphisms per locus) and rate of polymorphism (>13%) relative to other markers tested.
X-linked markers tested using UNPHASED.
Out of 32 ISSR markers, 15 markers produced clear, distinct and scorable bands with an average of 5.47 bands per marker.
The test produced mundane lessons as well.
Seven other tests produced similarly small but significant results.
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