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Of 48 markers tested on the reference Houghton (H) strain, one marker failed to amplify for unknown reasons.
Out of 79 markers tested on panel 2, 49 (62%) amplified one or two alleles per locus while the remaining 30 (38%) markers amplified more than two bands for at least one genotype (see Figure 1B).
Markers tested on this expanded dataset were assessed by a number of criteria, including sensitivity at 98% specificity, AUC, and mean normalized serum marker levels in specific subsets of cases and controls (Table 3).
Overall, 146 out of the 209 (69.9%) markers tested on G. armourianum produced successful assays.
When only cultivated cassava was considered, 40 out of 79 markers tested on panel 2 were polymorphic, corresponding to a frequency of 50.6%.
PIC values ranged from 0.08 to 0.90 with an average of 0.57 (Additional file 2: Polymorphism status of SSR markers tested on 22 parental genotypes).
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The markers were tested on one population of B. alba (30 individuals) and one population of the closely related B. pilosa (30 individuals) in China.
Class 3 events are defined by strains in which LOH is observed for all markers tested, including the marker on the opposite side of the centromere.
All other phenotypic markers tested including expression of CD57 on CD8+ T-cells and CD28 expression on CD4+ and CD8+ T-cells did not correlate with CD4 count change during TI.
Unsurprisingly, each of the markers tested had a significant impact on PFS in univariate analysis.
Of the 11 COSII markers tested, 10 were successfully amplified on the first attempt.
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