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Exact(9)
Future research should concentrate on specific indica markers targeting the identified insertion in the Pi33 zone.
Rather than using metabolite markers, it will therefore be important to develop DNA-based markers targeting the genes underlying the differential response of a given metabolite.
Although genetically linked markers were suitable for screening, DNA markers targeting the polymorphisms that define the functional and non-functional alleles of Pi2 and of Pi9 would greatly enhance the efficiency and accuracy of genotype selection in MAS breeding programs.
To fine-map dep, a total of 600 F2 plants generated by crossing GLSL15 with Koshihikari, as well as 7652 F3 plants generated from F2 lines with informative recombination points, were genotyped using SSRs and newly developed insertion/deletion (indel -based and derindel -based andlifiederivedorphicleavednce (dCamplified et al.[2002]) markers targeting the polymorphiccation of the gene.
The ApoSense family is a group of low molecular weight amphipathic apoptosis markers targeting the cell membrane of apoptotic cells.
Consequently, it can be argued that all the SNPs identified might be exploited in the future as markers targeting the specific desirable phenotype for assisted selection.
Similar(51)
It is not known yet whether ACP052 and API66 markers target the same gene or linked duplications.
Pi2-LRR is a CAPS marker targeting the LRR motifs of the coding region of Pi2.
Fig. 1 An InDel marker targeting the promoter region of Pi9. a Physical location of the identified InDel region in the Pi2/9 locus.
Fig. 2 A CAPS marker targeting the LRR domain of Pi2. a DNA polymorphisms corresponding to the eight amino-acid differences in between Pi2 and Piz-t.
Based on the multiple sequence alignment of 26 sequences of the promoter region of Pi9 and its alleles, primers 9-Pro-F/9-Pro-R were designed to develop a marker targeting the InDel polymorphisms among the Pi2/Pizt, Pi9, and non-Pi2/Piz-t/Pi9 alleles.
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