By comparing the captured mRNAs from each experiment, we identified a number of markers specific to VTA dopaminergic projection neurons.
Genome-wide TSS usage has been detected across many human cell-types in the FANTOM5 consortium data set (using cap analysis of gene expression; CAGE 36,37 and by the Roadmap Epigenomic Consortium using chromatin markers specific to TSSs38.
Many groups are exploring such techniques; some are hunting for genetic markers specific to viable embryos, while others are pursuing less invasive analyses of the substances that a viable embryo secretes.
The subcellular organelles, i.e., nuclei, mitochondria, microsomes, and cytoplasm, were isolated by centrifugation and verified for their authenticity by determining the markers specific to cellular organelles.
These 34 STS primer pairs were further used for screening markers specific to individual chromosomes of H. villosa using a complete set of disomic addition lines.
"In the past, we have tried to look in the blood for differences between healthy people and cancer patients," says Carbone. "We've had some success, but it's not as specific as we would like". So researchers are now looking for markers specific to tumor cells, and then determining whether those markers are detectable in blood.
Development of antigens specially designed for the identification of serological markers specific to acute infection and of IgG anti-HEV specific to the convalescent phase of infection would greatly facilitate accurate identification of active, recent and past HEV infections.
Markers specific to a native keratocyte phenotype such as keratocan and aldehyde dehydrogenase 3A1 (andH3A1) and those specific to a fibrotic phenotype such as α-smooth muscle actin (αSMA) and collagen type III were examined.
Nevertheless, the use of the internal transcribed spacer (ITS) region in the molecular analysis might have resulted in a low detection rate of arbuscular mycorrhizal fungi (see ref. 26 for more), and it is likely that arbuscular mycorrhizal fungal communities would have displayed spatial structuring when analyzed using DNA markers specific to arbuscular mycorrhizal fungi.
Random amplified polymorphic DNA (RAPD) assays were applied on 34 fungal strains isolated from strawberry and other host plants, in order to detect polymorphism to consequently identify and isolate molecular markers specific to Botrytis cinerea.
This work investigated in 3D the effect of TGF-β1, zone-specific growth factors, optimum matrix stiffness, and adding nanofibers on the expression of chondrogenic markers specific to the superficial, middle, and calcified zones of articular cartilage by the differentiating human mesenchymal stem cells (hMSCs).
