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Chi-square analysis (χ test) was carried out to identify markers showing high levels of segregation distortion.
The total DNA and the Alu 247 bp markers also remained significantly different, with all four markers showing high ROC values.
These results suggest that the most plausible demographic scenarios are compatible with individual markers showing high differentiation or height covariation in Pygmies (according to the overlap of simulated and real PI n and CI n distributions).
A total of 100 markers showing high repeatability were screened, and the robustness of PCR was again confirmed for 129 F. × ananassa lines, 119 of which were previously investigated polymorphisms identified using CAPS markers (Supplementary Table S2).
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Besides, the markers showing high-cross transferability across millet and non-millet species can be utilized for genetic enhancement of orphan crops using the marker-related genetic information of foxtail millet genome.
For 1D the heterogeneity was due to two markers showing higher retention.
In the present study, we tested the subset of 21 EST-SSR markers showing higher level of transferability [ 13] and polymorphism in H. chilense lines across genera for phylogenetic inference.
Conclusion: The screen using all 4 markers showed high sensitivity in all age groups without increasing the false-positive rate.
The nucleotide sequences of these markers showed high similarity to the genome sequence of chromosomes 3 and 11 of 'Golden Delicious' apple.
There was some inter-laboratory variability in read counts, but overall the results of the laboratories were comparable in that highly expressed markers showed high read counts and less expressed markers showed lower counts.
In the study of Lossos and colleagues [3] evaluating 36 genes, our markers showed high absolute univariate z scores.
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