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Among them, 107 markers showed polymorphic PCR bands between the parents and they were potentially used for characterizing the alleles existing among the 151 F2 plants.
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To further unify those four linkage groups, 17 RFLP and 49 SSR markers from the four linkage groups were selected to screen polymorphic between the parents of a cross CMS HA89 × RHA280, resulting in four RFLP and 25 SSR markers showing polymorphic.
Between 'Nakateyutaka' and 'YI-0311 'YI-0311didate polymorphic (11.9%), 10 candidate non-polymorphic (8.3%), and 14.9candidate probable polymorphic (9.9%) markers showed polymorphism.
Although KASPar assays were developed for SNPs identified to be polymorphic between ICP 28 and ICPW 94, only 71.4% (1154) markers showed polymorphism in the tested genotypes.
Although these markers may show polymorphic alleles in other genetic backgrounds, they should possibly be excluded from the genome panel.
Only four out of 100 SSR markers from the Gramene database (http://www.gramene.org) showed polymorphic differences between Wonseadaesoo and Junam varieties in our preliminary test for marker survey.
However, the second batch of HAU-InDel-prefixed markers showed the lowest polymorphic rate (4.88%).
Six of the 22 polymorphic markers showed significant association with GY (at varying P values of 0.05 and 0.01) under water-stress.
We found most polymorphic markers showed mainly two reproducible alleles in parent NL94.
The polymorphic markers showed a PIC value in the range of 0.08 to 0.86 with an average of 0.43 (Table 3).
The faecal DNA from 30 captive giant panda was used to test whether the polymorphic markers showed highly responsive and could be applied to faecal DNA.
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