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The markers show high PIC values and therefore will be useful for cultivar identification, taxonomic studies, and genetic mapping.
Slow mutation rates in the mitochondrial DNA point to efficient DNA repair mechanisms [ 31– 33]; however, cnidarian nuclear allozymes and microsatellite markers show high heterozygosity indicative of high mutation rates [ 33– 33].
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Conclusion: The screen using all 4 markers showed high sensitivity in all age groups without increasing the false-positive rate.
The nucleotide sequences of these markers showed high similarity to the genome sequence of chromosomes 3 and 11 of 'Golden Delicious' apple.
There was some inter-laboratory variability in read counts, but overall the results of the laboratories were comparable in that highly expressed markers showed high read counts and less expressed markers showed lower counts.
In the study of Lossos and colleagues [3] evaluating 36 genes, our markers showed high absolute univariate z scores.
TBARS and hydroperoxides (lipid peroxidative markers) showed high lipidperoxidation.
Chi-square analysis (χ test) was carried out to identify markers showing high levels of segregation distortion.
Although only a relatively small sample set was used to evaluate their variability, 16 markers showed high levels of polymorphism.
The total DNA and the Alu 247 bp markers also remained significantly different, with all four markers showing high ROC values.
These newly developed microsatellite markers showed high amplification success even though their polymorphism levels within populations are not very high, especially in the small populations.
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