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In osteogenic medium Von Kossa staining showed calcium deposition although osteogenic markers remained unaltered.
In osteogenic medium Von Kossa staining showed calcium deposition, although osteogenic markers remained unaltered, as assessed by qPCR.
Acquisition of the solid pattern was associated with a loss of immunolabeling for MMTV proteins (data not shown) by neoplastic cells, but the expression of terminal differentiation markers remained unaltered.
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Other markers remained unchanged.
Other ER stress markers analyzed remained unaltered, including PERKpThr980, ATF6, IRE1α-pSer724, spliced Xbp1 and Grp78 (Supplementary Fig S3A G).
Indeed, while old R6/2 mice (P104-P110) with established neurodegeneration and striatal atrophy present an increase in Glial Fribrillary Acidic Protein signal (GFAP, an astrocyte marker), GFAP reactivity remained unaltered in symptomatic R6/2 mice (P60 80) indicating that HD symptoms start before any astrogliosis.
The differentiation potential and self-renewal capability of the ES cell colonies decreased rapidly during the initial 40 hours after LIF removal, yet the expression level of ES cell markers remained relatively unaltered for up to 80 hours [ 18].
In WAT, expression of white fat markers (e.g. Cpt1, Prkaa2) remained unaltered between WT and Enpp1 −/− mice with a HFD (Fig. 4G,H).
We also show that infection of the developing brain, which is rich in NSCs, results in reduced expression of doublecortin (DCX), a marker that identifies young/immature neurons, while the glial precursor and mature astrocyte marker, glial acidic fibrillary protein (GFAP) expression remained unaltered.
We looked at some mitophagic markers (i.e., PINK1 and PARK2) and we found that they remained unaltered during myogenesis.
In contrast, Sox2 expression remained unaltered and could be detected together with the NSC marker Nestin for more than 35 passages (Fig. 5S V; Fig. S10 and data not shown).
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