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Details of the molecular structure of iPP and sPP, such as the hand of individual helical stems and setting of chains, are accessible by crystallographic considerations which make use of molecular markers provided by the α-α and α-γ epitaxies in iPP or by direct visualization by atomic force microscopy.
The use of a high number of SNP markers, provided by deep sequencing of a selected group of cultivars, enabled us to analyse in detail the temperate japonica group, and in particular, the genetic relationship of several Spanish cultivars with other groups from different countries.
The high marker density reflects the high degree of recombination in the mapping populations as well as the large number of markers provided by our genotyping method.
Although over 1,000 human miRNAs may be encoded in human genome, the potential therapeutic markers provided by miRNAs for a diverse spectrum of diseases are still unexplored.
Conclusions: The new markers provided by this study will substantially increase the resolution for detailed studies in phylogeography, population genetics, and parentage analysis.
A new set of EST-SSR markers, provided by Crouzillat et al. (Table 1), was also tested on the following Coffea species: C. canephora, C. heterocalyx Stoff., and C. pseudozanguebariae.
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Staining experiments were performed as previously described by Guenin et al. [ 8] Briefly, isolated larval AMC from embryos at stage 10-17 were incubated with various primary antibodies: MR1A mouse anti-Prospero at 1 4 dilution, rat anti-Elav at 1/1000 (a neuronal marker; provided by A. Giangrande), and rabbit anti-phosphohistone H3 at 1/1000 (a marker for mitotic activity; SIGMA).
We applied a simple method to computationally deconvolute gene expression profiles from composite samples, based on the signal provided by marker genes.
A large collection of new genetic markers was provided by this ESTs collection.
Monomorphic SSR markers were provided by Dr. Michael McMullen at the University of Missouri (Columbia, MO).
These BAC-end derived SSR markers kindly provided by Dr. Bertioli were also subjected to polymorphism analysis (Additional file 2).
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