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Round cells are specific markers of virus infestation.
This discrepancy may be explained: 1) by the choice of pp65 Ag assay for guiding antiviral therapy, which may have modified CMV viral load to be detected by plasma PCR; 2) the two methods target different markers of virus replication, detecting free virions in plasma and virus protein in PBL, with different levels of sensitivity [ 17- 21].
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There is interest to identify and confirm risk factors of severe disease and poor outcome and to evaluate laboratory markers of virus-specific immunity as surrogate markers of disease and recovery.
Infected cells were identified using a rabbit antibody (Orab1) that binds the viral NS2 protein (a marker of virus replication) and an Alexa-568 conjugated secondary antibody.
5' Rox- CAC ATC GAT AGA TCA AGG TGC CTA CAA GC BHQ2 3' AM-F and AM-R: Generic forward and reverse primers respectively binding to target sequences in the Influenza A matrix gene, enabling amplification of the matrix RNA as a marker of virus genome copies 20 pmol/µl each.
The biological significance of reassortments between genetically similar viruses may be arguable, but the frequency of reassortment events is an important marker of virus endemicity in a region.
Immunohistochemical studies with the antibody against the Ad hexon protein, the classic marker of virus particles produced in cells [ 55], showed the expression of the viral hexon protein in tumor tissues treated with Ad-cycE, but not in the control group treated with AdGFP (Fig. 5b, panels iii and iv), indicating de novo synthesis of Ad-cycE viral late proteins.
To this end, we analysed patients' peripheral blood samples to measure the gene expression levels of the biomarker IFI27, an established marker of virus-induced immune response [3].
One is to explore different genetic markers of the virus that are known to be associated with severe influenza.
One is to explore specific genetic markers of the virus that are known to be associated with severe influenza (e.g. the PB1 gene) [6], although the absence of a known marker, as was for example the case in a novel swine-origin influenza A (H1N1) virus (S-OIV), does not necessarily indicate that the virus is benign [7].
However, virus structural proteins either recombinant or natural help in establishing the cause of infection and are considered as important protein markers of JE virus.
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