Everolimus also decreased levels of total choline (Cho) in RIF-1 tumours, which is another marker of viable and proliferating cells, in this case reflecting membrane turnover.
For assessing human prostatic cell strain proliferation, trypan-blue exclusion was used as a marker of viable cells 9 12 days after treatments.
Further research is needed to develop new diagnostic tests that are able to quantify messenger RNA, a marker of viable, replicating organisms, rather than chlamydia DNA or ribosomal RNA, and use these new tests when re-testing people within 4 weeks after treatment.
Recently, bacterial DNA measurement in blood samples has proven to be a reliable, non-invasive, and probably more sensitive surrogate marker for translocation of viable bacteria in cirrhosis [ 48, 60].
The NAA signal provides a marker of the number of viable neurons [ 69].
As the CLL cells can lose their surface markers at death, the numbers of viable CLL cells were normalized to the total number of cells in the sample.
For each marker from each sample, the percentage of viable cells positive for that marker was determined.
Furthermore, we show that UCP measurements are viable markers of nutritional status from single void urine samples and that it is unnecessary to collect complete 12 hour samples (as done by Wolden-Hanson and colleagues, [38]).
The primary marker used was Pfg377, assumed to be a female-specific protein, and anti-α-tubulin II, which reacts with both sexes, albeit at different intensities, was taken as a secondary marker to provide a denominator of viable, intact gametocytes of both sexes.
The difficult challenge is to validate the correlation with marker uptake and the presence of viable hypoxic cells.
