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Case 102 (a clear-cell tumor) had two supernumerary markers of unknown origin in three metaphases of the 45 cells analyzed.
We have used this procedure to generate aptamers against cell surface markers of unknown identities on embryonic stem cells (ESCs; Iwagawa et al. 2011).
In total, 438 sires were genotyped for 580,961 SNPs that were distributed over chromosomes 1 to 28, two linkage groups (LGE22C19W28_E50C23 and LGE64) and the two sex chromosomes, along with a group of 7883 markers of unknown location.
Studies testing whether systemic inflammation might lie on the causal pathway between aberrant fetal and post-natal growth patterns and later cardiovascular disease have been inconclusive, possibly due to the use of single markers of unknown predictive value.
The best biomarker based on the GA/SVM wrapper approach alone is Fam134b, possibly a missing link between pluripotency and some standard surface markers of unknown function processed by the Golgi apparatus.
In the ES cell context, Golgi-mediated processing and/or transport of two cell surface markers of unknown function in pluripotency, SSEA1 (stage-specific embryonic antigen 1) and AP (alkaline phosphatase) may be impaired; both SSEA1 and AP are localized in the Golgi, to be transported to the cell surface.
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In fact the American Type Culture Collection (ATCC) describes the karyotype of this cell line as carrying 5 marker chromosomes of unknown origin, which supports this finding.
However, as any other genetic association analysis, DEFB1 −44C/G may be only a surface marker of some unknown real genetic marker of sepsis in linkage disequilibrium.
Consequently, the state of articular cartilage differentiation with respect to markers of hypertrophy is unknown in patients suffering from familial chondrocalcinosis.
Because statistics based on windows of several markers were applied across regions of unknown linkage disequilibrium between groups of markers, creating permutation thresholds was not feasible for this study.
Finally, two polymorphic markers significantly associated with PHE were identified: marker 354 (Solyc08g082350.2.1), which encodes for a protein of unknown function, and marker 4365 (Solyc11g010170.1.1), encoding for a LanC-like protein2, which is involved in the modification and transport of peptides in bacteria [ 38].
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