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PARP and α-tubulin were detected as markers of nuclear and cytosol proteins, respectively.
We used lamin-B1 and IKB-α, as markers of nuclear and cytoplasmic fractions, respectively, as previously reported.
We measured cell-free DNA, two markers of nuclear DNA, and two markers of mitochondrial DNA (mtDNA) at the 0, 12, and 24 h time points.
Recently, phylogenetic markers of nuclear genes were deciphered and successfully applied for reconstructing phylogenetic trees across diverse Cypriniform species [ 22– 25].
Tissue-specific binding patterns of TFs could explain why c-Jun and Jun-D were not identified as mtDNA binders in many studies and instead, were frequently used as markers of nuclear localization.
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Primary antibodies were from: Transduction Laboratories (p27, metablastin/stathmin and CDK2), Santa Cruz (p27 C19, p27 N20, CDK4, vinculin, Skp2), Clontech (Zs green polyclonal antibody), Sigma (Op18/stathmin), Upstate (RhoA), as marker of nuclear extracts we used an anti-HMG2A kindly provided by Prof. G. Manfioletti.
PIPKIγ_i4 staining co-localized with SC-35, a marker of nuclear speckles, but not nucleolin.
Earlier studies have assessed oxidative stress in tissues by immunostaining of 8-oxoG, a marker of nuclear DNA damage (Nakae et al. 2005).
Nevertheless, the localization of Ce-GnRHR to the nucleus provides an exceptional molecular marker of nuclear growth as germ cells progress through the gonadal arm prior to fertilization (Fig. 4).
Microsatellite DNA markers of the nuclear genome were used for genotyping the sampled trees.
(A and B ) FLIP experiments and BI values for the indicated markers of the nuclear envelope during early anaphase, in the cells of indicated genotype.
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