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At the same time, markers of neuron lineages (β3 Tubulin and GFAP) decreased in these cells.
Whereas S-100β is a marker of astrocyte damage, NSE and MPB are markers of neuron and white matter (myelin) damage, respectively.
Both lines contained an obvious CD133+ subpopulation that displayed neurosphere-like tumor spheres, nonadherent growth and asymmetrical cell divisions yielding cells that expressed markers of neuron and glia differentiation.
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Our understanding of how mammalian sensory circuits are organized and develop has long been hindered by the lack of genetic markers of neurons with discrete functions.
"People were claiming that they had [stem cell-derived] neurons, because they were expressing beta-3 tubulin which is one of the markers of neurons, but when I looked at them they were like fibroblasts, not really differentiating with axons, neurites, and synapses," says Stoppini.
After 7 days, neuro-MSCs changed morphology and expressed markers of neurons (MAP2, NSE), astrocytes (GFAP) and oligodendrocyte progenitor cells (Olig1, A2B5, O1 and O4) (Fig. S5).
Further immunohistological experiments with other markers of neurons, astroglial and oligodendroglial cells are required for the identification of a possible selection of a subpopulation of radiation- and apoptosis-resistant SVZ/OB cells.
To determine if there were also differences in the ability to generate the three main cell types of the CNS, differentiated NS were scored for immunoreactivity for markers of neurons (Tuj1), oligodendrocytes (O4) and astrocytes (GFAP).
Staining of 20 clones for markers of neurons (TuJ1), glial cells (GFAP) and smooth muscle cells (SMA) revealed 12 clones (63%) positive for all three lineage markers, 3 clones (16%) positive for TuJ1 and GFAP, 3 clones (16%) positive for SMA only, and 1 clone (5%) positive for TuJ1 only (Figure 8).
All of the neurospheres gave rise to cells with the molecular markers of neurons or astrocytes.
The purity of motor neurons were shown in Supplementary Figure S4A using specific markers of neurons (MAP2) and astrocytes (GFAP).
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