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In the present study, the symptoms of recurrent lung infections (considered as markers of infected pulmonary embolism) were found in 24.9 % of patients with the final diagnosis of LDIE.
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For the assays, we isolated EBV-infected cells from PBMCs using magnetic beads-conjugated antibodies targeting the surface markers of the infected cells.
The plasma levels of cytokines and chemokines in the acute phase did not correlate with peripheral blood WBC counts or any other inflammatory markers of EV71-infected patients (data not shown).
Adenoviral vectors applied in Figure 1 were constructed to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) as markers for the identification of infected cells.
Interestingly, ProDH and OAT were selected by a VIGS-based forward genetic screen by looking for genes regulating non-host resistance [ 10], and the participation of these enzymes in disease resistance was inferred from the reduction of HR markers in infected tissues of ProDH- or OAT- silenced plants [ 9, 10].
This result suggests that genome-wide expression profiling of PBL from BTB animals can be used to enable the identification of suitable transcriptional markers for the detection of infected animals within herds and augment current surveillance strategies in countries where control programmes have been implemented [ 21, 22].
25 CADM1 expression and CD7 loss have recently been identified as highly sensitive molecular markers of HTLV-1-infected cells.
Because the EVC family may be membrane-associated proteins (Fig. 4), 30 the present study provides us with the possibility that EVC expressions might be useful cell markers of HTLV-1-infected T-cells for future clinical purposes.
Virus was produced as described below and ZSGreen1 protein was used as a marker for the selection of infected cells.
Mass spectrometric analysis of peritoneal lavage fluid revealed a marked increase in the levels of oxidized amino acids and of F2-isoprostanes (sensitive and specific markers of lipid oxidation) in infected animals.
Expression of this construct is driven by a U6 promoter and contains a blasticidin S marker gene for positive selection of infected cells.
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