Our results suggest that differentially phosphorylated proteins could be considered as more promising markers of differentiation for NB than differentially expressed proteins.
To determine the effects of GO on the osteoblastic differential potential, two markers of differentiation, ALP and MM, were analysed in hMSC-TERT cells after exposure to appropriate conditions for the induction of differentiation.
The advantage of this technique is that when the cells are thermo-switched to 37°C they exit the cell cycle and are able to differentiate, expressing cellular markers of differentiation.
To determine whether the BrdU+ cells differentiated, sections were co-labeled with markers of differentiation.
Either platelet derived growth factor (PDGF) or retinoic acid (RA) prevented neurosphere formation from dispersed secondary neurospheres and resulted in cells with a differentiated morphology (Supplementary Figure 1C) and expressing markers of differentiation.
Canonical early and late molecular markers of differentiation described in the adipose lineage exhibited similar profiles in muscle-differentiating progenitors.
Calzadilla, P. et al. N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes.
EGFRvIII+ cells are associated with other stem/progenitor markers while markers of differentiation are found in EGFRvIII- cells.
Some BMMNCs exhibited the presence of α-MHC and troponin I, markers of differentiation into cardiomyocytes.
We also assayed for additional markers of differentiation such as TROPONIN2 (TNNI2) and MYOGENIN (MYOG) in cells under normal growth conditions and under differentiation conditions.
Further immunostaining and quantitative real time PCR was used to quantify various markers of differentiation in the neuronal lineage.
