Collectively, these observations in primary tumors and metastases suggest that clinically relevant subgroup of prostate cancer can be defined by exploring markers of cell differentiation and cell proliferation.
We established an immortalized human prostate stromal cell line with retained markers of cell differentiation and α1-adrenergic receptor expression.
We next examined the time course of cytokine-induced translocation of GRP78 to the plasma membrane in murine MIN6 cells by flow cytometry as compared to markers of cell death (Fig. 2).
To study intrinsic matrix biologic cues, human endothelial cells were seeded onto acellular slices and analyzed for markers of cell health and inflammation.
The quantitative content of next-generation sequencing data was analyzed and confirmed using qPCR, to find that miRNAs are markers of cell status.
New markers of cell activation may be of interest.
Evolving technologies have provided high throughput approaches to investigate potential gene and protein signatures, and methods to measure newly discovered markers of cell death and immune responses.
This is probably also true in markers of cell cycle arrest, which are reasonably expressed when kidney cells are already distressed [25].
Yet, the diagnosis is often difficult and is based on the combination of clinical manifestations, biological markers of cell lysis, and suggestive abdominal CT scan findings.
Multiple assays have been adopted to enable the homogeneous measurement that can serve as markers of cell viability, cytotoxicity, and apoptosis.
We engineer a strain of Saccharomyces cerevisiae with fluorescently labeled markers of cell cycle progression, and apply our new model to two image datasets we collected from the strain, as well as an independent dataset of different markers.
