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However, no differences in markers of basal oxidative damage were observed in the present study between wild-type and fat-1 mice at 1 year of age.
Molecular analysis of IKOαβ epidermis revealed that K5 and K14, markers of basal keratinocytes, were expressed throughout the basal and suprabasal layers, while suprabasal markers K1 and K10 were delayed or weakly expressed.
To assess the keratin expression pattern further, application of antibodies to keratin 5 and keratin 18, markers of basal and luminal cells, respectively, was also conducted.
Cytokeratins 5 and 15 (KRT5/ KRT15), markers of basal cells in prostate glands, show uniform downregulation in all metastatic tumors, confirming the absence of basal epithelial cells.
We used markers of basal keratinocytes (Keratin 14, p63), spinous layer (Keratin 10), granular layer (Loricrin, Filaggrin), and hair follicles (β-catenin, Lef-1) in E18.5 skin sections.
Furthermore, this expression was strictly associated with cytokeratins 14 and 17, which are markers of basal and parabasal cells in normal laryngeal epithelium (Van der Velden et al, 1997).
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Both have previously been described as markers of basal-like breast cancers [54], [55].
Gene expression microarray analysis showed that KB1P tumors expressed markers of basal-like breast cancer, for example p63 and keratin 5, compared with the KP tumors [ 12, 32].
Over the recent years there has been controversy about their role in breast cancer and their suitability as markers of basal-like phenotype.
Phospholipase D1 tends to be overexpressed in tumours that express high levels of CKs 5/17, markers of basal-like tumours, and others have shown basal-like tumours are frequently associated with poor prognosis.
Keratin 14 is a specific marker of basal cells.
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