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We observed that the peritoneal macrophages from mice in HF fed condition express more strongly the proteins encoding for the MR, Dectin-1 and CD36, established markers of alternative M2 macrophage activation (Figure 2A).
Macrophages recruited to the peritoneum by F. hepatica show all the hallmarks of alternative activation; by 5 days after infection, the expression of markers of alternative activation, Fizz1, Arginase 1 and Ym1 are up-regulated and remain so for the subsequent 3 weeks [6].
However, there was no effect on the upregulation of expression of mRNA for markers of alternative activation (not shown).
We confirmed that AM with upregulated expression of markers of alternative activation also had enhanced expression of TNF- α by intracellular staining in F4/80+ macrophages.
Unexpectedly, we did not observe any reduction in the upregulation of expression of markers of alternative activation by AM from animals treated with anti-IL-33.
We found evidence of significantly upregulated expression of mRNA for Ym1, although in this in vitro setting other markers of alternative activation were not increased in parallel.
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Henson, J. D. et al. DNA C-circles are specific and quantifiable markers of alternative-lengthening-of-telomeres activity.
Stein, M., Keshav, S., Harris, N. & Gordon, S. Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation.
The state of macrophage activation was determined by mRNA expression of iNOS as a marker of classical activation (M1) and Fizz-1 as a marker of alternative activation (M2).
Interestingly, the expression of Fizz-1 as a marker of alternative activation or M2 phenotype [23], [24] was found only in macrophages from double mutant mice, suggesting that both TLR4 and TLR9 are required to prevent alternative macrophage activation during infection.
SENP1 was included as a potential marker of alternative lengthening of telomeres in the hTERT negative, but immortalized cells and it was a significant biomarker in our study.
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