Markers rs3737672, rs3765986, rs2791519, rs2791498, rs1882753, rs772602 and rs1517899 were genotyped, six two-marker haplotypes of adjacent markers were constructed and the haplotype distributions were compared between case and reference populations.
Similarly to human and mouse, zebrafish pancreatic tumors showed a highly aggressive behavior and propensity for metastatic spread, as demonstrated by an increased expression of mesenchymal markers and infiltration of adjacent organs (Fig. 2 and Fig. 3), which ultimately led to the death of carrier animals.
Yap et al.[ 50] were the first to model a map as a directed graph, with nodes representing mapped markers and edges defining the order of adjacent markers.
Detection was based on either the PIP of individual markers, or the PIP summed over intervals of adjacent markers.
We created LD plots using marker-pair associations of adjacent SNPs within a chromosome, adjacent SNPs within a haplotype block, and adjacent SNPs within genes.
In the final marker ordering, 51% of adjacent map bin pairs are separated by a single recombination event in the cross, and 94% are separated by three or fewer recombinants in each parent.
If two adjacent markers were in complete LD one of the markers was deleted, so that r of any pair of adjacent markers was less than one.
QTL detection can be based on several criteria, including marker effect, variance and PIP, either for each marker individually or summed up over an interval of adjacent markers.
An alternative procedure for marker selection that takes MAF and distances of adjacent markers into account was proposed by Wang et al. [ 27].
In polygenic scenarios, larger QTL were detected when detection was based on the PIP of individual markers than when based on the PIP summed up over a group of adjacent markers.
