Details about primer sequences, GenBank accession number, annealing temperature for PCR, PCR product size and locations of the 63 markers located in different BAC clones are summarized in Table 3. Ta: annealing temperature for PCR.
King et al.26 revealed two loci of the alliinase markers located in a distance of 6.9 cM26.9
Ostrich absence of heteromorphic sex chromosomes, unique sequences or markers located in the ostrich W-chromosome.
When in a defined workplace, a person is carrying out a labor operation, the IR camera registries the motions of the markers located in each hand.
There were 4 non-target fragments on chromosomes 1, 4, 7 and 12, among which the fragments at the end of the long arm of chromosomes 1, 4 and 7 were detected by adding 60 SNP markers located in the large interval without markers.
Then, the markers heterozygous for BC3F1-22F01 and 60 newly added markers located in the large interval without markers were used to genotype the 90 recombinant lines, from which a line named BC3F2-2B09, heterozygous at SNP2-SNP4 but homozygous Kongyu 131-type at SNP1 and with the highest RRPG, was selected (Fig. 7).
The second set was 63 markers located in 20 starch biosynthesizing genes including AGPase (AGPL1, AGPL2, AGPS1 and AGPS2), granule-bound starch synthase (GBSSI and GBSSII), starch synthase (SSI, SSIIa, SSIIb, SSIIc, SSIIIa, SSIIIb, SSIVa and SSIVb), starch branching enzyme (BEI, BEIIa and BEIIb) and starch debranching enzyme (ISA1, ISA2 and PUL) (Additional file 1: Table S3).
We selected and genotyped 66 different SNP markers located in, and around, the 9 genes.
In BXD, there are 307 genes with maximal LOD scores on markers located in Qrr1.
Three to five SSR markers located in the distal ends of each linkage group were selected to screen a fosmid library developed from inbred line 9930.
Our data showed that the positive BACs screened by each of 61 (54%) of the markers located in a single contig.
