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Furthermore, loxP site-mediated removal of selection markers left a 45 bp scar within the targeted exon that can be traced in mRNA without exon skipping.
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Addition of OHT at a later stage can induce excision of drug-resistant markers, leaving a loxP site at the GFP DsRed junction.
Transient expression of Cre recombinase was then used to remove the drug selection marker, leaving a single LoxP site between exons 21 and 22 and not affecting the LoxP site between exons 12 and 13.
We have developed a system for mycobacteria which features both the advantages of the use of antibiotic resistance markers for gene disruption experiments and the ability to efficiently rescue the marker leaving an unmarked mutation on the chromosome.
For this purpose a minimum genetic distance of 0.1 cM was chosen as a trade-off between the number of markers left for the analysis and the LD minimization.
Specifically, we considered the genotype of a marker that differed from the genotypes of immediately adjacent markers left and right of it as highly improbable due to chiasma interference [35] [37], and removed the markers with the deviant genotype(s) from the dataset.
When a short candidate segment is trimmed there may not be enough markers left to provide sufficient information to be confident of IBD.
They function more like forensic evidence, markers left behind by the dead, the lost or the emotionally absent as proof of their existence.
These are all markers left by someone.
"You don't know where you are, there are no markers left," Stoner said.
The number of markers left in the different segments of the colon was counted, and the GITT was calculated based on the following formula: GITT = M + f × D D where M is the total number of markers left; D is the number of markers ingested each day; and f is the fraction of the daily markers selected for the provision of transit.
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