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Immunopositive cells for all markers (labelling indices) were counted or scored for each individual crypt.
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Unlike nuclear markers, labeled SPION, internalized by macropinocytosis, accumulate within endosome/lysosome compartment.
Further, glial involvement was determined with Iba1 and GFAP markers, labeling microglia and astrocytes, respectively (Fig. 4).
These markers label the same population.
Correlation of markers' labeling scores with clinical outcome variables was computed by Spearman rank correlation.
The results of simultaneous staining of renal tumors with multiple markers labeled with Fluolid dyes are shown in Figure 1.
A recent study in mouse suggests that different subplate markers label subplate cells of different birth dates.
Each SSR marker was arranged to eliminate overlap with other SSR markers labelled with the same fluorophore in each multiplex set.
Finally, markers labeled ZEST were designed from sequences first reported in [ 47] and are microsatellites isolated from the zebra finch EST Genbank entries.
Four SSR markers labelled with different fluorophores were chosen for even distribution on each chromosome on the basis of previous results.
As expected, in young adult kidney cortex, pericyte markers labeled afferent arterioles (arrowheads, Figure 3A, a), and peritubular capillaries (arrows, Figure 3A, b-c).
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