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We combined large-scale population calcium imaging in the hindbrain with simultaneous high-speed recording of the moving tail in animals where specific markers label glycinergic inhibitory neurons.
These markers label the same population.
A recent study in mouse suggests that different subplate markers label subplate cells of different birth dates.
In wt late stage 17 embryos, both markers label SJs and trace the outlines of SPG, which make continuous contacts with their neighbors to seal the CNS.
This conclusion follows from the analysis of the ability of ascites cells to internalize TAMRA-labeled DNA, presence of CD34 marker and CD34+ cell engraftment, which clearly shows that both markers label the same TISC subpopulation.
Remarkably, tubules with a strong PNA-lectin staining showed relatively weak Ksp-cadherin expression and vice versa, suggesting that both markers label different parts of the distal tubules with different intensities.
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Unlike nuclear markers, labeled SPION, internalized by macropinocytosis, accumulate within endosome/lysosome compartment.
Further, glial involvement was determined with Iba1 and GFAP markers, labeling microglia and astrocytes, respectively (Fig. 4).
Correlation of markers' labeling scores with clinical outcome variables was computed by Spearman rank correlation.
Immunopositive cells for all markers (labelling indices) were counted or scored for each individual crypt.
However, like all cell death markers, labeling is transient because the affected cells and their corpses ultimately disappear.
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