The onsets of the lagging markers were represented with almost identical amplitudes in all age groups.
Estimating that β-actin mRNA occurs in 500 2,000 copies per cell, we suggest that these negative markers are represented in our samples by at most 1 copy for every 10 cells, because their amplification required 11 18 cycles more than β-actin mRNA.
Linkage group with notation 'n' represents reference map, SSR markers are represented by red colour.
Each LG and markers are represented with a vertical bar and transverse line, respectively.
Nondiagnostic markers are represented by diagonals with areas under the ROC curves close to 0.5.
Eighteen positions contain two markers, at three positions three markers are represented, and one position is occupied by four markers.
In aggregate, a total of 5,295 typable T. gondii genetic markers are represented on the array, and accuracy for those SNPs carried forward is >95%.
These markers are represented by the integers 1, 2,..., n, with a plus or minus sign to indicate the strand they lie on.
These markers are represented by 985, 634, and 44 SNP-harboring PstI tags and 1010, 951, and 40 PA variants from the PstI-MseI, PstI-MspI, and PstI-MluI libraries, respectively.
