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These crossovers are often missed in segregation analysis, since covering chromosome ends with suitable markers is difficult for the presence of telomeres and associated repeats (Sonnenberg et al. 2016).
The marker density of the map is not sufficient for fine-scale mapping and the reproducibility of RAPD markers is difficult.
Identifying underlying causative genes or even tightly linked markers is difficult for genetic resistance to MD, as is the situation for other complex traits.
However, there are many laboratory situations such as bird (Tobalske and Dial, 1996) or insect (Ellington, 1984) flight where attaching markers is difficult or may affect the outcome of the experiment, and this is where video photogrammetry provides a viable option for obtaining 3D data.
Similar(56)
A truly robust biomarker has not been discovered and exogenous markers are difficult to use in critically ill patients.
Secondly, the procedures for developing such markers are difficult, complex, and high-cost.
However, RAPD markers are difficult to transfer to other mapping population.
Some studies with genetic mapping figures not available or studies whose markers were difficult to place on the current map were discarded.
Due to their dominant nature, AFLP markers are difficult to use for inferring genetic parameters of populations, particularly ancestral population sizes, split times, and migration rates.
Because of the relatively thin cement mantle and the rectangular and voluminous proximal stem design, the visibility of the cement markers was difficult and only 26 hips could be measured at 2 years.
These stress markers are difficult to measure in large surveys where direct examination of the stress response (e.g. measuring cortisol) is problematic due to the circadian rhythms shown in these stress hormones and the rapid sampling required in order to measure baseline versus activated levels.
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