Of 98 gastric cancer patients, 96 patients displayed at least one marker informative of LOH status.
With only 32 markers informative for ancestry, the efficiency of the analysis is ∼70%.
In order to evaluate the proportion of mappable EST-SSR markers we have calculated the percentage of heterozygous markers informative for all combinations between 15 sexually compatible citrus genotypes, currently used or susceptible to be used in citrus genetic programs.
This is important as the dynamics of antibody acquisition and maintenance vary based on exposure intensity, thus the degree to which some serologic markers are informative of exposure will likely vary in these contexts.
40 In isolation, no other genetic marker was informative of a future diagnosis in patients with UPIA.
When a marker is informative of two minor alleles (Fig. 1A, "informative genotype 1"), this corresponds to the frequency of the heterozygous individual in the population.
Conversely, when the marker is informative of only one minor DNA allele (Fig. 1A, "informative genotype 2"), the frequency of the matching genotype includes the frequency of the corresponding homozygous individual in addition to all of the possible heterozygous individuals who carry the observed allele together with any of the DIP opposite haplotypes.
In these cases, the absence of a marker is not informative of the evolutionary process, only of the choices or technical proficiency of scientists or effectiveness of available protocols.
The identification of informative markers of key events can be greatly enhanced by the use of whole genome techniques such as gene expression assessments [using microarrays or preferably mRNA counting techniques such as SAGE or ultra-high throughput sequencing (Mardis, 2008)], or proteomic assessment [using mass spectrometry based techniques (Latterich et al., 2008)].
CIN phenotype classification was performed by calculating the LOH ratio of the informative markers of the seven polymorphic microsatellite sequences, independently from the UNC5C and DCC loci.
