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Tumor targeting is of high clinical and biological relevance, and major efforts have been made to develop molecular imaging technologies for visualization of the disease markers in tissue.
Non-homogeneity of synaptic markers in tissue surrounding plaques can present challenges in analysis.
When possible, which it should be for reporters like GFP and lacZ, the authors should use fluorescence detection to colocalize markers in tissue sections.
We have developed a quantitative multiplex-methylation specific PCR (QM-MSP) to quantitate cumulative methylation of these markers in tissue and serum samples.
A recent study showed that embryonic prostate mesenchyme acts to induce prostate lineage markers in tissue recombinants using several types of non-prostate postnatal epithelium [ 49].
Generally, measurements of tumor biological markers in tissue extracts by ELISA require relatively large amounts of fresh-frozen tumor material as well as adequate storage capacities for tumor samples.
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Other potential applications of MB are the quantification and detection of genomic disease markers in tissues and living cells.
The theme of markers continues into chapter 21, which discusses pharmacodynamic (PD) markers in tissues, including direct and indirect PD analysis of angiogenesis inhibitors and multi-targeted agents.
Rather than focusing on circulating markers alone, the authors examined markers in tissues, including lungs since they are a common site for secondary infections.
The hypothesis was that topical use of C. verbenacea could control the pathogens associated with periodontal destruction, and modulate the pattern of immune-inflammatory markers in tissues presenting periodontitis.
Mice are immunostained for GFP (green) to mark K3-expressing neurons and interneuron markers (magenta) in tissue.
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