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Detection of other methylated markers in stool DNA improved sensitivity with little effect on specificity.
The detection of CRC-specific DNA markers in stool has been studied extensively.
The presence of tumor markers in stool can be attributed to leakage, exfoliation, or secretion [ 88].
The third, most recent, study evaluated a test of DNA markers in stool [ 12].
Here, we investigated the stimulating effect of spermidine as PCR facilitator for detecting tumor-specific methylated markers in stool DNA.
A new method of CRC screening that tests DNA markers in stool (sDNA) may be a promising tool for screening in the future.
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We have also investigated the utility of one of the hypermethylated genes (EN1) as a diagnostic marker in stool and serum DNA samples of colorectal cancer patients.
Other DNA, RNA, and protein molecules under study tend to track the same markers studied in stool.
Although considerable efforts are being made to identify DNA, RNA, and protein markers present in stool to develop novel detection methods, only three clinically stool-based tests for CRC diagnosis are currently available: gFOBT, fecal immunochemical test (FIT), and detection of vimentin methylation.
Druker, who led the development of the blockbuster blood cancer drug Gleevec, plans to survey the field for a range of possible detection targets, which could include circulating tumor DNA in blood or other molecular markers in urine, stool, or saliva.
Our panel of markers is the first demonstration in blood of advanced precancerous colorectal lesion detection to show better performance than other marker panels tested in stool only (Tables S3 and S4).
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