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To explore the underlying mechanisms, the expression level of apoptosis, inflammation and autophagy-related markers in brain tissue were detected with kits or by western blot.
We had difficulties finding conditions that were compatible with both GCB and synaptic markers in brain sections and thus we performed this double labeling on primary cortical neurons from mouse embryo.
3) Was double labeling of nuclei with the different markers in brain sections was confirmed by z-stacking of the sections?
To this end, we counted the number of cells expressing neural markers in brain sections of E11.5 wild-type and Tg Dyrk1a embryos.
The early TBI and SCI studies noted co-localization of autophagy markers in brain and spinal cord regions showing high levels of neuronal cell death, leading to conclusions that autophagy may be contributing to neurodegeneration (20, 28).
Lack of cell bodies immunoreactive for hα-syn and lack of transcriptional AAV markers in brain regions rostral to the MO not only indicate specific transduction but also rule out the possibility that translocation of viral vectors rather than neuron-to-neuron transfer of hα-syn may underlie caudo-rostral spreading of the exogenous protein.
Similar(54)
CD133 has been suggested as CSC marker in brain tumors [21], breast [3], colon [22], pancreatic [23], liver [24], skin [25], prostate cancers [26] and Ewing's sarcoma [27].
To date, the relevance of Ang‐2 as a potential prognostic marker in brain malignancies has not been evaluated.
It is suspected to be a CSC marker in brain [ 9, 46] or colon [ 12, 47] tumours, although this view has been challenged [ 48, 49].
It seemed reasonable that telomerase expression might be a useful new prognostic marker in brain tumours, based on numerous studies in the literature on its prognostic implications in other tumour types, like breast, colorectal, endometrial, cervical or ovarian cancer.
CD133 has been identified as a cancer stem cell marker in brain (Singh et al, 2003, 2004), prostate (Collins et al, 2005), colorectal (O'Brien et al, 2007; Ricci-Vitiani et al, 2007) and pancreatic (Hermann et al, 2007) cancers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com