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Fifteen ISSR markers generated a total of 3773 bands, out of which 3770 were monomorphic among all the clones.
The primer pairs of 45 selected SSR markers, including 4 FAES and 41 FVES markers, generated a total of 158 peaks in the 129 strawberry lines that were tested (http://vim.kazusa.or.jp/Strawberry/).jp/Strawberry/
The DArT subset of markers generated a consensus map with a single gap between 15 and 20 cM (4HS) and five gaps between 10 and 15 cM on chromosomes 3H, 4H and 5H (the set of 'bPb' markers alone had an additional gap of this size on 4H).
Of these, 880 SNP markers generated 1 2 1 clustering of the mapping population, like an F2, 1,695 SNP makers generated 1 1 clustering, like a test-cross, and 2,588 SNP markers generated a single cluster indicating no polymorphism between the parents.
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CISH is based on the enzymatic amplification of DNA markers, generating a colorimetric image that can be visualized by a bright-field microscope and does not require a fluorescence detection setup.
When a marker generated a negative map distance or a large 'jump' value in goodness-of-fit test, the marker was removed, and map calculation was continued to construct a first-round map.
When a marker generated a negative map distance in the map or a large "jump" value in goodness-of-fit, which is the normalized difference in chi-square value before and after adding the marker, the marker was removed, and map calculation was continued to construct a first-round map.
When Cas14 binds to its target DNA sequence – a cancer gene or a gene in infectious bacteria – and starts cutting DNA, it will also cut the DNA linked with the marker, generating a fluorescent signal.
The compound nature of this marker generates a high level of polymorphism that is suitable for identity testing.
We co-injected P hsp-16.1 ::venus with selection markers and an injection marker, generating a parent Ex strain tm234 tm246 tmEx2677. Approximately 16,000 P0 animals were treated by UV/TMP and cultured for positive-negative selection as described above, resulting in 12 transformants.
The high number of DArT markers generated in a single assay not only provides a precise estimate of genetic relationships among genotypes, but also their even distribution over the genome offers real advantages for a range of molecular breeding and genomics applications.
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