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rufipogon populations, incorporating markers from previous GoldenGate 384-SNP sets and selective SNPs from whole genome re-sequencing data.
Design of the Illumina Infinium 6 K SNP chip for rice, referred to as the Cornell_6K_Array_Infinium_Rice (C6AIR), includes 4429 SNPs from re-sequencing data and 1571 SNP markers from previous BeadXpress 384-SNP sets, selected based on polymorphism rate and allele frequency within and between target germplasm groups.
Cross-amplification in other salmonid species using PCR conditions that were optimized for rainbow trout was determined (Table 1) to be similar to markers from previous publications [ 33].
We identified markers from previous studies that occur in genetic map locations adjacent to high FST genomic regions in our comparison (Table S3).
In previous studies, polymorphism rates of SSRs were ∼2.5 3.5%, and the rate of polymorphism markers from previous studies was 3.55% in the current study.
e other types include sequence tagged allelic restriction sites (31), erythrocyte antigens (10), single strand conformational polymorphisms (6), serum protein markers (5), phenotypic observations (2) and sequence tagged site (2) markers from previous bovine maps[4,5,12].
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Individual LGs including all markers available from previous studies were calculated for each mapping population first.
A set of 178 genetic markers selected from previous versions of the PI 161375 × T111 melon genetic map (mainly RFLPs [ 21] and SNPs [ 24, 31, 41, 42]) were used to anchor 845 BAC clones from our genomic library to the genetic map [ 26].
The map also included 184 markers from the previous maps (BA and A2), 124 new markers that had not been mapped in the previous maps (GenBank accession no; DQ865460-865479, DQ868392, EF112607-112700, and50284-459473) and 50 markers that were developed and genotyped by other authors [ 29, 40- 44, 49] 49].
The purpose of this current study was to bring together marker data from previous mapping studies, add almost 200 new markers and develop a high-density consensus reference genetic map for Lu.
In each round, the flanking markers from the previous round were retyped to confirm the validity of the recombinants.
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