All 106 resulting loci evaluated with the SSCP technique were placed in the linkage map, along with a further 11 Indel based markers evaluated for segregation with agarose gels.
Therefore, these polymorphisms could be useful as markers for meiotic segregation of MUC2 minisatellites during study of MUC2-related inheritable diseases.
Markers were tested for segregation distortion by the chi-square test.
Nevertheless, the possibility that the markers are uninformative for segregation analysis in the future couples requesting PGD for hemophilia A exists.
Spo0J is an initiation marker for DNA segregation in B. subtilis cells at the onset of DNA duplication.
Polymorphism was established between the parents before a marker was evaluated for segregation across the genotypes of 272 siblings in the F1 mapping population.
SNP markers were initially tested for segregation distortion using the chisq module of TMAP [ 38].
Markers were not tested for segregation distortion prior to map construction because distorted regions may be present in the genetic maps (Xu et al. 1997), especially those involving multigenerational populations.
Chi-square analysis (χ test) was carried out for each mapped marker (except for markers with segregation profiles 5, 8 and 9).
