CD31, a monoclonal mouse antibody (BD Biosciences, San Jose, CA, USA), was used as a marker for rat endothelial cells.
CD105, a polyclonal rabbit antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), was used as a marker for rat stem cells.
Moreover, the cells took up fluorescently labelled acetylated LDL (Fig. 5), a specific marker for rat coronary endothelial cells (6).
Two-color staining revealed that a substantial number of cells with cytoplasmic HMGB1 expression were also positive for ED1, a marker for rat macrophages and dendritic cells.
The number of blood vessels that were identified as positive isolectin B4, the marker for rat endothelial cells, was counted at × 40 magnification (n = 5 for each group).
Although further studies are required to establish the usefulness of KS expression in human polyneuropathy, our study demonstrates that KS is a good marker for rat EAN.
Tissues from rats subjected to 120 minute MCAO with 2, 10, 24, or 48 hours of reperfusion were double-labeled for RECA-1, a marker for rat endothelial cells, and analyzed for perivascular Aqp4 intensity.
As for the rat, a recently published report describes for the first time isolation and culture of cells that express markers specific for rat trophoblast stem cells (in the presence of FGF4) and their ability to differentiate into trophoblast giant cells (when FGF4 is removed from the medium) [ 40].
A more commonly used and reliable immunostaining marker selective for rat and human PSC (and absent in fibroblasts) is glial fibrillar acidic protein.
After 1 week the animals were killed, the temperature data retrieved from the iButtons, and the time of the morning temperature decline used as a primary phase marker for each rat.
