We chose these three markers for the preliminary classification of Coja haplotypes because they are located in the Tapasin, Coja-DBB1 and Coja-DMB2 loci, respectively.
With this method, only a small number of validated anchor markers are required for preliminary mapping, and the markers for fine mapping can be easily developed from detected polymorphisms from NGS in the target region.
Six polymorphic SSR markers spanning the Pi2/9 locus were used for preliminary and fine mapping of the R gene in Jefferson (Table1).
Results showed that applying the morphological characteristics as preliminary screening markers for the identification of induced tetraploid lines was feasible.
The expression of splice variants in these cells may contribute to the development of new diagnostic markers for such disorders, although a preliminary examination failed to reveal the expression of hDATΔEX6 in the brains of PD patients.
For chloroplast DNA markers, a preliminary screening test involving 14 rapidly evolving cpDNA regions [ 27] was conducted (ndhF-rpl32; prbA-trnH; psbD-trnT; psbE-petT; rpl32-trnL; rps16-trnK; trnD-rpoB; trnK-matK; trnL-trnF; trnQ-5′ rps16; trnS-trnC; trnS-trnfM; trnT-trnG; and trnV-ndhC).
The genotype and phenotype of all these BC2F2 individuals were compared and percent co-segregation for markers within the preliminary mapped locus was computed.
Markers 1, 4, 6, and 7 were used for preliminary screening (Table S4 and Table S5).
An attempt was made for preliminary small in silico validation with available markers of SGN database [ 14] using PERL script (Table 2).
