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Although they cannot be used to study (Delta varPsi _{text{m}}), they are good markers for mitochondrial morphology.
Meanwhile, an informal survey of seven families of children with cases currently pending in Vaccine Court revealed that all seven showed markers for mitochondrial dysfunction, dating back to their earliest medical tests.
We were able to reproduce this finding under our experimental conditions, as indicated by the fact that citrate synthase activity, PGC1-alpha and cytochrome c oxidase expression, markers for mitochondrial biogenesis [24]; [27]; [36]–[38], increased in the adipose tissue and skeletal muscle of CR animals relative to AL (Figs. 1A 1F).
This suggests that the surrogate fluorescence markers for mitochondrial mass and mitochondrial membrane potential can detect subtle phenotypes in cells with heterozygous mutations in genes that cause recessive phenotypes, as described here for Sucla2 (Fig. 1D).
It is well known that in healthy individuals exercise training stimulates mitochondrial biogenesis, indicated by an overall increase of markers for mitochondrial mass, like mitochondrial DNA (mtDNA) copy number and citrate synthase activity [ 14, 15].
For pea, we assayed marker enzyme activities also on membrane fractions obtained from fractionation of shoot microsomal membranes by a 10-step aqueous polymer two-phase counter current distribution [ 24]. Figure 1 shows the distribution of proteins and of markers for mitochondrial inner membranes, ER, Golgi apparatus, thylakoids and plasma membranes between the 10 fractions.
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Mitochondrial-localizing RFP (Mt-RFP) was used as a marker for mitochondrial localization.
This protein being highly expressed at the outer mitochondrial membrane [22] serves as a marker for mitochondrial mass [23].
Mitochondrial DNA (mtDNA) copy number was determined as a marker for mitochondrial density using quantitative RT-PCR as previously reported [46], [47].
When measuring NAO fluorescence as a marker for mitochondrial content, we found that mutants grisea and PaCox17::ble indeed contain significantly more mitochondria than the WT WTT: 100%, grisea: 160% [p<0.001 vs. WT], PaCox17::ble: 217% [p<0.001 vs. WT]) (Fig. 3B).
Ψm has been used as a marker for mitochondrial activity [ 37, 38].
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