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Bimpong et al. (2013) used 194 polymorphic SNP markers for mapping QTLs related to salinity tolerance.
GWAS can guild the genetic variations with the high-density SNP markers for mapping the regulatory locus of these traits.
Specifically, when developing new markers for mapping studies, e-PCR can be used to test potential primers in various ways before actually incurring the expense of oligonucleotide synthesis.
The Affymetrix sugarcane genechip was used to survey the SFPs with the GeSNP software between two sorghum cultivars that differ in the accumulation of fermentable sugars in their stems, with the objective to develop genetic markers for mapping purposes.
The major goal for plant breeding is to select the best progenies with the desired traits, and GWAS can guild the genetic variations with the high-density SNP markers for mapping the regulatory locus of these traits.
This was because earlier studies have used either SSR markers (maximum 264) or less than 300 SNP markers for mapping QTLs for heat tolerance whereas we have used more than 800 SNPs and 272 RILs to achieve a much higher resolution (Buu et al. 2014; Ye et al. 2012, 2015).
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Thus, researchers from different laboratories can use these markers for map development and comparative mapping.
This left 463 (4.6% of the total) RAD loci, plus the nine morphological markers, for map construction.
Markers for map construction were isolated from our genomic libraries [ 7].
In total, 94 seedlings from the mapping progeny (MJ × MO) were genotyped with molecular markers for map construction.
These genes can then be used to generate transgenic plants or can serve as molecular markers for map-assisted breeding [ 7].
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