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Additionally, the ability to evaluate differentiation has been challenged by a lack of markers for differentiation.
Next, we examined markers for differentiation after 1, 3 and 5 days of BMP treatment.
There is a growing interest in the use of anti-glycan antibodies as markers for differentiation and stratification of CD.
We conclude that our CSF flow-adapted 2D-DIGE protocol is valid especially in comparing samples with high differences in total protein and suppose that GFAP and sAPPα/β have a high potential as additional diagnostic markers for differentiation of BM from VM.
Lymphoid expression of several markers for differentiation status were examined (supplementary Table S5), for line 131 no differences from controls were observed for any of the lymphoid tissues and antibodies tested (data not shown), suggesting that for this line the status of the B and T cells (with respect to the antibodies used) is not modified by the presence of the transgene.
Genetic markers for differentiation at these scales included partial sequences of mitochondrial cytochrome- c-oxidase subunit I, and an array of nuclear DNA microsatellite loci [ 39].
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To assess the rate of spontaneous differentiation in these cells, expression of the cornified envelope precursor involucrin, a commonly used marker for differentiation, was analysed.
Closer inspection of many antibody-stained colonies revealed that there were rare transition regions that simultaneously expressed OCT4, the gold-standard marker for pluripotency, and full-length MUC1, which appears to be a marker for differentiation.
These findings classify MS4A8A/MS4A8B as a novel marker for differentiation in the gut.
Increase in cytokeratin 8/18 expression was used as the marker for differentiation (Peehl et al, 1993; Hsieh et al, 1995).
We believe that an application for 1,5-AG as a marker for differentiation between other MODY subtypes or in young type 2 diabetic subjects will be found eventually.
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