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Although work in different animal species revealed distinct molecules that might be used as characteristic markers for IVD or specifically nucleus pulposus (NP) cells, the validity of these markers for characterization of human IVD cells remains unknown.
The cells were either pelleted for future research or labeled with florescent markers for characterization.
Given the trend toward increased use of SNP markers, it is of interest to compare the performance of these two types of markers for characterization of the same set of individuals in a model crop plant, maize.
These sequences could be potentially useful for the development of molecular markers for characterization of the genetic variability and population structure of B. alternatus throughout its geographic distribution.
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Currently, there is no gold-standard marker for characterization of CACs.
The number of obligate breaks/deletions, among other factors, depends on chromosome length and number of markers used for characterization.
We have demonstrated that insertions-deletions can be effectively utilized for QTL mapping applications in non-model organisms and INDELs can serve as useful alternatives for SNP and microsatellite markers, especially for characterization of genetic architecture in multiple crosses and large pedigrees.
However, the morphology of these presumptive monocyte-lineage cells varied across the two cases, with predominantly large mononuclear cells in case 1 and small elongated cells in case 2. The combinations of cell surface markers used for characterization of inflammatory cells do not enable clear distinction between DCs and cells of macrophage lineage.
The development of reliable and informative markers for the characterization of OS in humans is thus highly important.
Similarly, SSRs are the most sought after DNA markers for germplasm characterization in several tree species like avocado (Gross-German and Viruel [2013]), Psidium guajava (Sitther et al. [2014]), Olive (Trujillo et al. [2014]), Tamarix (Terzoli et al. [2014]) and Prunus (Khadivi-Khub et al. [2014]).
As a proof-of-principle we chose to analyze sorted NCAM+EpCAM−, NCAM+EpCAM+ (containing putative MM stem- and MM-derived progenitor cells, respectively) in comparison with NCAM− HFK cell populations as NCAM and EpCAM are important surface markers for our characterization system.
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