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The aim of our study was to find and characterize markers for cell proliferation and dedifferentiation in cultured chondrocytes.
We first examined markers for cell proliferation.
The first fluorescence channel enables detection of stained nuclei, resulting in markers for cell locations.
To obtain pure cultures of STRO-1+, CD271+ and CD133+ cells, we used these markers for cell sorting.
Levels of the G1 cyclin Cln2 and the mitotic cyclin Clb2 were also monitored to provide markers for cell cycle progression.
These cocktails included antibodies against 2 to 5 phenotypic markers for cell population gating (e.g., CD45, CD33), up to 3 antibodies against intracellular signaling molecules, or against surface markers for an 8-color flow cytometry assay.
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Therefore, cleavage of PARP and fodrin facilitates cellular disassembly and also serves as markers for cells undergoing apoptosis.
Release of LDH from cells into the surrounding medium is a typical marker for cell death.
Therefore, supernatant LDH of cultured HAECs is detected as a marker for cell injury [36].
(1) Holdenrieder S, Stieber P, Bodenmuller H, Fertig G, Furst H, Schmeller N, et al. Nucleosomes in serum as a marker for cell death.
Figure 5a and b shows that, compared with fresh air, CS exposure rendered cells less capable of reducing resazurin, a dye commonly used to determine the metabolic activity of cells as a marker for cell viability [23].
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