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Markers flanking a putative QTL were selected as cofactors and the selected marker was used as genetic background controls.
If genotypes of markers flanking a missing data point do not match, the missing data point is left missing.
Using coordinates of public markers flanking a bin, physical (in base pair) and genetic (in cM) lengths of a bin were determined.
Physical and genetic lengths of a chromosomal bin were calculated by subtracting the physical (bp) and genetic (cM) coordinates of public markers flanking a bin where GLS resistance QTL resided.
Whereas no genetic linkage between other Boron exclusion and efflux transporter genes was detected, strong linkages were evident to genetic markers flanking a cluster comprising five predicted NIP genes explaining most (95%) of the phenotypic variation observed in the segregating Medicago populations.
Map position of each new marker was also assigned on the Vitis reference map of Doligez et al [ 30] by comparing the position of the pair of reference markers flanking a new marker in 'Chardonnay', 'Cabernet Sauvignon', 'Bianca', and/or '20/3' with the same reference markers placed on the integrated map.of Doligez et al [ 30].
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On the other hand, when markers flank a recombination event, additional markers in the region will increase resolution of the recombination position.
The association of a rearrangement at markers flanking 5q21 with a bad prognosis is suggested by the curves, but the correlation is not statistically significant (P=0.15).
Additional file 4: Validation of 18 Competitive Allele-Specific PCR (KASP) markers designed from Illumina iSelect markers flanking QTL in a RL4452/'AC Domain' population.
These results highlight the usefulness of BSA based on markers flanking QTLs, as an efficient tool for saturation of targeted regions, opening the possibility of future marker-assisted selection for these traits.
QTL intervals were deduced from centiMorgan map distances between SNP markers flanking QTL peaks of a given PHS resistance trait (GI, SI or FN).
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