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Design: Subjects had infrared light-emitting diode (LED) markers fixed on their temporomandibular joint, greater trochanter, and lateral malleolus.
Boundary conditions of the model are defined by the three-dimensional coordinates of 43 external markers fixed to bony landmarks of the hand and forearm and tracked with an optoelectronic motion capture system.
The 3-D motion analysis system can simultaneously record (with high resolution) the instant 3-D coordinates of the multiple markers fixed on a vibrating cable; this distinguishes it from other experimental systems used in vibration studies, in which the vibration of only one single point can be recorded during each individual testing.
Wind-blown snow is expected to partially cover the gear but GPS markers fixed to the corners of the site will help locate it again.
Cells were subsequently stained for surface markers, fixed and permeabilized in Fix Buffer and Permeabilization Buffer (eBioscience) according to manufacturers' instructions and stained with IL-17A-PE (eBioscience) or matching isotype control.
After 6 h of stimulation, cells were washed, stained for the surface markers, fixed, permeabilized using the BD Cytofix/Cytoperm™ Kit (Becton Dickinson) and stained intracellularly using the appropriate fluorochromes.
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The marker fixed on the support frame was used as a reference for measuring the total stretching of the two canvases.
In our study we ignored the non-segregating SNP markers, as these markers are fixed in the simulated population and show no variation.
Those markers were fixed to allow more markers to be added on the linkage map by reducing the LOD threshold to 3.0 (except for 2.0 to link two segments of LG 6b).
The 6 additional genes (COL11A2, RXRb, RING1, PRIM2A, COL21A1 and RPS18) were mapped at their most likely location relatively to markers of this preliminary framework map, considering the distance between framework markers as fixed.
When I made the fields smooth but at a very slight angle, so the rains would run off, and set the boundary markers, and fixed the posts deep in the earth, so they would not fall down?
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