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DNA samples were PCR-genotyped with SSLP markers evenly spaced across the zebrafish genome.
Genotyping was completed on 367 autosomal and 21 X-chromosome microsatellite markers evenly spaced throughout the genome (average spacing of 9.2 cM).
A total of 63 polymorphic micro-satellite markers, evenly spaced on each chromosome, were used to screen the offspring at each generation.
The originality of our study is the large number of common markers, i.e. 29 common markers evenly spaced along the three chromosomes.
Information about polymorphic microsatellite markers for the BC (118 markers evenly spaced throughout the genome with an average inter-marker distance of 20 cM) was retrieved from Ensembl Genome Database (http://www.ensembl.org v.50-62 v.50-62
The availability of a comprehensive linkage map, with markers evenly spaced across the genome, will facilitate the systematic searches necessary to identify genes affecting traits of economic importance, with the potential to incorporate marker information into the animal selection process using marker assisted selection (MAS) [ 27, 28].
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All loci were equidistant and markers were evenly spaced between the non-marker loci.
For chromosomes 11 15 we considered two cases (a) markers were placed with the same density as on the first 10 chromosomes, or (b) markers were evenly spaced every 2 cM.
Three hundred markers were evenly spaced across the genome.
The markers were evenly spaced across the entire autosome.
An implicit assumption is that the probability of a QTL being located in any of the bins is equal, which is met when markers are evenly spaced.
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