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CEA, CYFRA21-1 and CA125 were measured using validated assays and performance of markers evaluated for different pre-diagnosis time groups.
All 106 resulting loci evaluated with the SSCP technique were placed in the linkage map, along with a further 11 Indel based markers evaluated for segregation with agarose gels.
Mreps was used to screen for simple sequence repeats (SSRs), with markers evaluated for polymorphism using 20 diploid (AA) M. acuminata accessions contrasting in resistance to Mycosphaerella leaf spot diseases.
Of the 300 SSR markers evaluated for polymorphism and mode of segregation in the B493 × QAL population (see Additional File 1 - Table S2), 170 (56.7%) were monomorphic and 66 (22%) polymorphic, whereas 28 SSRs (9.3%) did not produce amplicons and 36 (12%) yielded ambiguous band patterns that did not allow their inclusion in the previous classes.
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No biological or serologic marker evaluated for routine blood donor screening in France was associated with these donations.
This was consistent with the fact that 70 accessions from Asia and Africa did not show any polymorphism for the markers evaluated.
The decrease of LD as a function of distance between markers was evaluated for pairs up to 4 Mb apart using markers with minor allele frequency above 0.01.
During the study, patients were monitored before each cycle for medical history, physical evaluation, clinical benefit, serum chemistry and tumour markers, and evaluated for toxicity.
Combinations of the 5 markers were evaluated for their association with <32 weeks spontaneous preterm birth.
Defense gene expression, often using the PR1 and PBZ1 genes as markers, was evaluated for a large number of cases (37/55).
The CSSL19 line was backcrossed to the Nipponbare parent and F3 or F4 lines were genotyped using microsatellite markers and evaluated for nitrogen-induced susceptibility to GUY11.
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