But not all of these markers are established in every pathology laboratory, and sometimes it is desirable to rely on more than one or two markers.
Synteny using common molecular markers was established in order to develop a genetic linkage map taking advantage of the information provided by previous eggplant genetic linkage maps.
Because of the high degree of paracentric inversions present in this species, the order of the genetic and physical markers already established in many cases differs from that occurring in the Gd-H4-1 lineage (Schaeffer et al. 2008), which emphasizes the importance of establishing physical markers in the sequenced lineage and correctly aligning their genomic and cytological maps.
Interestingly, a conserved synteny of ftr82/83 and a few neighboring markers was established in zebrafish, stickleback and medaka, indicating that they were kept in a more stable genomic context than other fintrims; it is also worth noting that in the medaka, these genes have retained the six-exon structure.
Mendelian inheritance of the EST-derived markers has been established in two international reference mapping families of P. monodon, and mapping of these markers is in progress.
The prognostic significance of specific single genomic markers is well established in neuroblastoma, and has led to their implementation in current risk assessment.
As no stem cell marker has been established in zebrafish or human, verification of the model results still awaits future work in this field.
Using the developed inducible promoter, a counterselection marker MazF was established in the ARAi system for C. cellulolyticum.
The senescent phenotype (growth arrest, cell enlargement and acquisition of the SA- β-Gal marker) was clearly established in all cells after 48 72 h, as already published.
The relative phase specificity of each marker was also established in the ovary by double labelling and confocal microscopy to ensure that there was no significant coexpression of putative markers of adjacent cell cycle phases.
