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The availability of large-scale genome-wide association (GWA) studies using 500,000 or more single nucleotide polymorphism (SNP) markers equally distributed across the genome, now facilitates identification of susceptibility genes in polygenic and complex phenotypes, e.g. type 2 diabetes or MI [9] [17].
Microarrays probing for 500,000 or 1,000,000 SNP markers equally distributed in a single human genome now enable such studies.
Genomes consisted of one single chromosome of a length of 9 cM with 10 biallelic markers equally distributed over the chromosome.
The final set comprised 6000 markers, equally distributed along the chicken genetic map (Additional file 3: Figure S2) with the notable exception of GGA16, W and of linkage group E64, for which the assembly is still incomplete.
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For a whole-genome scan, 221 markers were equally distributed over all 29 bovine autosomes, with an average distance of 13.7 cM.
In this study the markers were equally distributed across the chromosomes.
In each of 100 repetitions, subsets of the markers (9 to 252 by 9 grad) were either randomly selected (random sampling) or sampled in such a way that the selected markers were equally distributed across the genome (stratified sampling) [ 12].
This observation suggested that by choosing markers that are equally distributed across the genome, it is possible to reduce their number compared to randomly distributed markers while achieving the same level of precision in assigning inbreds to subgroups as well as estimating MRD.
Of the 168 markers, 86 (51%), equally distributed over nine LG, were common to the pea reference genetic map [ 50], including 74 SSR, 11 RAPD and one morphological marker (Pl).
Expectedly, ABCG2, an established EV membrane marker co-localized with the apical GPI marker (Figure 2A 2F); in contrast, the basolateral marker VSVG was equally distributed in the cell membrane and did not display an exclusive co-localization with ABCG2, an EV membrane marker (Figure 2G 2L).
For genetic mapping purposes, a much denser marker set, preferentially equally distributed over the genome, is required.
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