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We previously reported the isolation of Z and W linked markers, developed a genetic sex test and sequenced a 3.2 kb region of the sex chromosomes [ 28, 29] in Pogona vitticeps.
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This RH panel will be an invaluable resource for mapping gene based markers, developing a complete marker scaffold for the whole genome sequence assembly, fine mapping of markers and functional characterization of genes and gene networks present on the D-genome.
For using SSR markers of one species in the evaluation of other species, our study recommends first to screen a large number of SSR markers developed in a particular species and then identify the subset of most reliable markers that amplify the expected amplicons in the other species.
We used cross-species microsatellite markers developed for a close relative, A. thaliana, to detect genetic structures at a fine resolution.
These are the first microsatellite markers developed for a Quillaja Molina species.
Markers developed from a 670-kb region on sorghum chromosome 7 (58.37–59.04 Mb) all cosegregated with the d2 phenotype.
In this paper, we describe the isolation and properties of 14 new polymorphic microsatellite markers, developed with a low-cost procedure and next-generation sequencing on T. superba.
Dixit et al. (2005) found that only ten out of 50 SSR markers developed from a sesame DNA library were polymorphic in 16 sesame accessions [ 7].
The EST-SSR markers developed herein represented a valuable resource for the genetic diversity analysis, genetic mapping and marker-assisted selection in radish.
Therefore, the set of genic-SSR markers developed here is a promising genomic resource.
Conclusions: The markers developed will be a valuable genetic resource for medium-throughput genotyping of Central American species of Begonia sect.
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