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We used primer pairs for SNP markers described at genome.wustl.edu or by Davis et al. (2005).
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For example, a marker described as pCMET.1003.N refers to CMET phosphorylated at residue 1003 localised in the nucleus.
Markers described in this manuscript are outlined in Table S3.
Immunohistochemical detection of the protein gene product 9.5 (PGP9.5), a highly specific pan-neuronal marker, was described at peritoneal lesions developing from surgically transplanted uterine horn segments in a rat model of endometriosis [ 138].
Details of genetic markers and balancer chromosome are described at Flybase (http://flybase.org).org
QTL and eQTL mapping was performed using GeneNetwork and a standardized set of 3795 genotyped markers (mapping algorithm and genotypes described at ; genotypes downloadable as a text file from ).
Markers were a subset of those described at http://naturalvariation.org/geno.html.org/geno.html
Several indirect and direct markers of alcohol exposure (see figure 2A) exist and have been described at length elsewhere (Bakhireva and Savage 2011).
Dysfunctional perforin expression has been described at HIV seroconversion [63], however, the impact of early and prolonged treatment on this marker remains unknown.
The genotypes at the sd1, Wx, Xa4 and Xa21 loci in each plant at different generations were determined with molecular markers as described in Table 1.
Results of these analyses on hES at passage 49 are shown in Fig S1. hES cells were characterized for pluripotency by staining for pluripotency markers as described [24].
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